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Giardia eIF4Es are incapable of complementing the function of S. cerevisiae eIF4E. S. cerevisiae yTHC strain YSC1180-7428777 was transformed with the Leu-selectable vector pGADT7′ containing cDNAs encoding yeast eIF4E, Giardia eIF4E1, and Giardia eIF4E2, respectively. Following selection on the −Leu plate under G418 selection, cells from individual colonies were serially diluted and applied to synthetic complete medium plates lacking leucine but containing doxycycline.
