FIG. 8.
Colocalization of Giardia eIF4E1 with m2,2,7GpppN cap, fibrillarin, and 16S-like rRNA. (A) Giardia WB cells stained with rabbit polyclonal antibody against eIF4E1, mouse monoclonal antibody against m2,2,7GpppN cap (top), and mouse monoclonal antibody against yeast fibrillarin (bottom). (B) Giardia WB trophozoites were fixed and stained with an FITC-conjugated 30-mer oligonucleotide complementary to the 3′ end of the Giardia 16S-like rRNA. Concentrated stains were visible in the nucleolus-like structures at the anterior ends of both nuclei.