FIG. 4.

Optical tweezer homing assay experiments. (A) Control experiment. At 0 min, CATs (c1 and c2) formed from the thicker tips of germ tubes (g1 and g2) of two macroconidia (a and b) are homing toward each other. At 3 min, the two germlings have been moved apart with the optical tweezers. At 42 min, the CAT tips are reorientating their growth back toward each other. At 90 min, the CAT tips have adhered and fused with each other. (B) CATs (c1 and c2) formed directly from conidia (a and b) of a cr-1 strain lacking cAMP home toward each other. At 0 min, the CATs are homing toward each other. At 7 min, the two CATs have been moved apart with optical tweezers. At 13 min, the CAT tips have reorientated their growth back toward each other. At 43 min, the CATs have adhered and fused. Bars, 10 μm.