Fig. 3.

Combination of MSC and MNC-QQ transplantations improved limb salvage rate in ischemic hind limb model mice. MSC (2 × 10⁴ cells/body) and the combination of MSC + MNC-QQ (1 × 10⁴ cells/body, each cell) were administered intramuscularly into the ischemic hind limbs immediately after surgery. (A) Representative images of ischemic hind limbs. (B) Necrosis scores of the ischemic hind limbs (0 = no change, I = nail necrosis, II = toe necrosis, III = foot necrosis, IV = knee necrosis, V = total amputation) measured on day 21 (N = 8). (C) The blood flow preservation rate in the affected limb was measured using a laser blood flow imager. Representative images from day 21 are shown. White arrows show the ischemic limbs. (D) (E) The blood flow ratio of the ischemic versus the contralateral hind limb was calculated on days 0, 1, 3, 7, 10, 14, and 21. (N = 8) Graphs show the mean ± SD. ∗p < 0.05, ∗∗p < 0.01. SD, standard deviation.