Figure 4.

Gene regulatory network inference from the single-cell RNA-seq data identifies 4 key regulators that modulate the critical cellular transitions to OCs

See also Figure S3.

(A) The cellular state transitions (tree) from the progenitors to the BMMs and the transcription factors underlying each of the edges, identified by SCDIFF2.

(B) The gene regulatory network reconstructed by the iDREM tool shows that Cebpd, Sox4, and Irf8 are regulating the gene expression dynamics between cluster 16 to cluster 12, the critical state transitions from the progenitors to BMMs.

(C) The log-normalized expression of the 4 predicted key regulators (Cebpd, Irf8, Sox4, and Klf4) are monotonically changing along with the trajectory.

(D) The expression changes of top predicted transcription factors along with the trajectory.

(E)Top differential genes associated with the critical cellular state transitions for OCs.