Table 1.
Summary of SPR binding kinetics data
| Nanobody ID | kon (1/s) | koff (1/Ms) | KD (M) | ka sem | kd sem | KD sem | n |
|---|---|---|---|---|---|---|---|
| LaG94-1 | 1.1E+06 | 1.8E-05 | 1.6E-11 | 3.0E+05 | 2.3E-06 | 4.7E-12 | 5 |
| LaG94-2 | 1.1E+05 | 3.0E-03 | 2.7E-08 | 1.4E+04 | 4.1E-04 | 4.8E-09 | 3 |
| LaG94-3 | 1.1E+07 | 1.5E-03 | 1.3E-10 | 1.5E+06 | 2.5E-04 | 2.9E-11 | 3 |
| LaG94-4 | 4.1E+05 | 1.5E-03 | 3.7E-09 | 4.5E+04 | 1.8E-04 | 5.9E-10 | 3 |
| LaG94-5 | 5.6E+06 | <5.0E-06 | <8.9E-13 | 7.8E+05 | n/a | 1.2E-13 | 5 |
| LaG94-6 | 5.2E+06 | 4.3E-04 | 8.3E-11 | 8.1E+05 | 5.9E-05 | 1.7E-11 | 3 |
| LaG94-8 | 5.5E+06 | 1.2E-03 | 2.1E-10 | 4.3E+06 | 7.5E-04 | 2.1E-10 | 3 |
| LaG94-9 | 4.5E+07 | 9.5E-04 | 2.1E-11 | 2.4E+07 | 3.7E-04 | 1.4E-11 | 3 |
| LaG94-10 | 3.9E+06 | 3.5E-05 | 9.0E-12 | 1.1E+06 | 9.9E-06 | 3.6E-12 | 5 |
| LaG94-12 | 1.3E+06 | 4.1E-04 | 3.0E-10 | 1.9E+05 | 5.3E-05 | 5.8E-11 | 3 |
| LaG94-15 | 3.6E+07 | <5.0E-06 | <1.4E-13 | 2.2E+07 | n/a | 8.6E-14 | 3 |
| LaG94-18 | 3.3E+05 | 3.1E-04 | 9.2E-10 | 9.2E+04 | 4.1E-05 | 2.8E-10 | 3 |
| LaTdT-1 | 2.8E+05 | <5.0E-06 | <1.8E-11 | 6.0E+04 | n/a | 3.8E-12 | 2 |
| LaTdT-2 | 1.3E+07 | 4.5E-05 | 3.5E-12 | 1.1E+06 | 1.4E-05 | 1.1E-12 | 2 |
| LaTdT-8 | 1.3E+07 | 4.8E-04 | 3.7E-11 | 4.4E+06 | 1.1E-04 | 1.5E-11 | 4 |
| LaTdT-9 | 2.1E+05 | 1.8E-05 | 8.4E-11 | 4.8E+04 | 1.0E-05 | 5.2E-11 | 4 |
| LaTdT-10 | 7.9E+05 | 4.1E-05 | 5.2E-11 | 1.5E+05 | 1.4E-05 | 2.1E-11 | 4 |
| LaTdT-38 | 1.7E+06 | 9.7E-05 | 5.7E-11 | 2.9E+05 | 1.8E-05 | 1.5E-11 | 4 |
| LaTdT-39 | 1.1E+06 | 6.3E-06 | 5.9E-12 | 4.8E+04 | 1.0E-05 | 5.2E-11 | 3 |
| LaTdT-44 | 1.1E+06 | 4.9E-05 | 4.4E-11 | 2.7E+05 | 1.2E-05 | 1.5E-11 | 2 |
| LaTdT-45 | 4.7E+05 | 1.3E-05 | 2.8E-11 | 4.4E+03 | 2.8E-07 | 6.4E-13 | 2 |
| GST-1 | 1.1E+06 | 9.2E-05 | 8.5E-11 | 1.2E+05 | 4.2E-06 | 1.0E-11 | 3 |
| GST-2 | 1.2E+06 | 7.9E-05 | 6.4E-11 | 4.3E+04 | 2.1E-05 | 1.7E-11 | 2 |
| GST-3 | 1.4E+06 | 4.8E-04 | 3.5E-10 | 6.0E+05 | 7.1E-05 | 1.6E-10 | 3 |
| GST-4 | 4.0E+05 | 1.1E-04 | 2.6E-10 | 1.5E+05 | 3.5E-05 | 1.3E-10 | 3 |
| GST-5 | 8.7E+05 | 1.1E-04 | 1.3E-10 | 2.3E+05 | 3.9E-05 | 5.7E-11 | 3 |
| GST-6 | 4.1E+06 | 6.1E-04 | 1.5E-10 | 2.2E+05 | 3.8E-05 | 1.2E-11 | 2 |
| GST-7 | 4.3E+06 | 7.6E-04 | 1.7E-10 | 7.8E+04 | 6.6E-05 | 1.6E-11 | 2 |
| GST-8 | 2.4E+05 | 3.1E-05 | 1.3E-10 | 9.8E+04 | 1.0E-05 | 6.9E-11 | 2 |
| LaMIgG-5 | 2.8E+05 | 1.2E-04 | 4.2E-10 | 1.4E+05 | 3.6E-06 | 2.1E-10 | 2 |
| LaMIgG-8 | 1.3E+05 | 1.6E-04 | 1.2E-09 | 2.8E+04 | 1.7E-06 | 2.8E-10 | 2 |
| LaMIgG-9 | 2.9E+05 | 1.8E-04 | 6.0E-10 | 3.3E+04 | 1.9E-05 | 9.3E-11 | 2 |
| LaMIgG-14 | 2.2E+05 | 4.7E-05 | 2.1E-10 | 1.6E+04 | 8.6E-06 | 4.3E-11 | 2 |
| LaRIgG-1a | 8.9E+05 | 3.4E-03 | 3.6E-10 | 1.4E+04 | 7.1E-04 | 9.6E-11 | 2 |
| 2.6E-03 | 2.6E-04 | 1.5E-04 | 2.9E-05 | ||||
| LaRIgG-2 | 3.6E+05 | <5.0E-06 | <1.4E-11 | 1.5E+05 | n/a | 6.0E-12 | 4 |
| LaRIgG-7 | 9.6E+05 | 5.1E-05 | 5.3E-11 | 1.2E+05 | 1.0E-06 | 6.6E-12 | 2 |
| LaGIgG-4a | 2.2E+06 | 1.1E-02 | 8.2E-10 | 1.9E+05 | 1.9E-03 | 3.6E-10 | 2 |
| 1.8E-03 | 3.3E-04 | 2.7E-04 | 3.0E-05 | ||||
| LaGIgG-12 | 3.2E+05 | 1.6E-04 | 4.9E-10 | 6.7E+04 | 2.6E-05 | 1.3E-10 | 3 |
a
2-state binding model.