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. 2024 Apr 16;47(5):100721. doi: 10.1016/j.bj.2024.100721

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© 2024 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Analysis of ^99mTc-UCMSC-EVs. (a) The radiochemical purifications of ^99mTc-UCMSC-EVs and ^99mTc-NaTcO4 were determined by iTLC on glass microfiber chromatography paper impregnated with silica gel and normal saline as the mobile phase. A purity of >99.9% of ^99mTc-UCMSC-EVs was achieved before the animal study. (b) Radioactive and UV HPLC chromatograms of ^99mTc-UCMSC-EVs were obtained. The radioactive and UV chromatogram peaks representing ^99mTc-UCMSC-EVs showed the same retention time. (c, d) The stability of ^99mTc-UCMSC-Evs was examined after incubation with PBS and FBS for 1 h, 4 h, and 24 h at 37 °C. Slightly decreased stability of ^99mTc-UCMSC-EVs at 24 h after incubation with PBS was found.