Fig. 4.

Impaired function of Zbtb24-deficient B1 cells in vivo. FACS-sorted peritoneal cavity CD19⁺B220^lowCD23⁻ B1 cells from control Cd19^Cre/+ and Zbtb24^B−CKO mice (female, 12 weeks of age) were adoptively transferred into Rag2^−/− recipient mice (female, 8 weeks of age) intraperitoneally (2 × 10⁵ cells/200 μl PBS/mouse). On D20 post injection, Rag2^−/− recipient mice were immunized with NP-LPS (20 μg/200 μl PBS/mouse) intraperitoneally. Blood was taken at indicated times and cells in peritoneal cavities (PeC) and spleens (SPL) were analyzed by flow cytometry on D27. A A schematic flow-chart showing the experiment setup. B, C Bar graphs showing levels of total (B and tIgM in C) and NP-specific (NP-IgM in C) IgM in sera of recipient mice on D10 and D20 (B) or D27 (C). D, E Representative offset histograms/bar graphs showing the percentages of CD19⁺ B cells and CD19⁺CD138⁺ plasma cells (PC) within gated B cells in the spleens (SPL, D) and peritoneal cavities (PeC, E) of recipients implanted with Cd19^Cre/+ or Zbtb24^B−CKO B1 cells. Each dot represents a single recipient mouse, and numbers below horizontal lines indicate P values. Data are representative of two experiments