FIG. 3.

Time course of replication-competent-nucleocapsid formation in transduced PTHs and Huh7 cells. Nucleocapsids from cytoplasmic lysates of PTHs and Huh7 cells, infected at MOIs of 125 and 50, respectively, with Ad-HBV1.3, were prepared at the indicated times, subjected to gel electrophoresis in native agarose gels, and blotted on nylon membranes. DNA and core protein were detected by chemiluminescence, using a DIG-labeled HBV DNA probe (upper gels) and a core-specific antiserum (lower gels). M, DIG-labeled DNA marker, with fragments sizes in kilobases indicated on the left; St, linear 3.2-kb HBV genome; rec. core, 5 ng of particles from recombinant core protein amino acids 1 to 149.