Table 2.

Relative biomass composition [% of AIR] of the pre- and post-fermentation residue

Condition	Arabinose	Galactose	Glucose	Xylose	HC	CC	Lignin	Acetate	Starch	U. maydis	Total
B73—U. maydis	2.5 ± 0.1	0.7 ± 0.0	2.5 ± 0.4	23.1 ± 0.7	28.9 ± 0.7	35.1 ± 0.6	15.8 ± 0.9	5.0 ± 0.3	0.5 ± 0.2	n.d	85.3 ± 1.7
B73 + U. maydis	2.5 ± 0.1	1.0 ± 0.1	2.8 ± 0.3	18.3 ± 0.7	24.6 ± 0.6	32.6 ± 1.2	16.4 ± 0.2	3.9 ± 0.2	0.8 ± 0.0	11.2 ± 1.4	89.5 ± 1.2
bm3—U. maydis	2.4 ± 0.2	0.6 ± 0.0	2.1 ± 0.3	23.6 ± 0.8	28.7 ± 0.8	35.4 ± 1.5	15.4 ± 1.2	5.0 ± 0.2	0.4 ± 0.2	n.d	84.9 ± 2.9
bm3 + U. maydis	2.2 ± 0.1	1.0 ± 0.1	3.0 ± 0.2	18.0 ± 1.0	24.2 ± 1.1	33.3 ± 0.8	14.2 ± 0.8	3.5 ± 0.2	0.7 ± 0.0	15.4 ± 1.5	94.6 ± 1.7
B73—Celluclast®	1.7 ± 0.2	0.9 ± 0.1	2.5 ± 0.6	18.2 ± 1.6	23.2 ± 2.2	32.2 ± 2.7	15.3 ± 0.7	4.4 ± 0.3	0.7 ± 0.0	12.6 ± 1.5	88.4 ± 1.8
B73 + Celluclast®	1.2 ± 0.1	0.6 ± 0.1	0.3 ± 0.3	14.7 ± 1.0	16.9 ± 1.2	27.5 ± 1.8	16.6 ± 0.9	4.5 ± 0.7	0.8 ± 0.1	24.1 ± 1.9	90.4 ± 2.1
bm3—Celluclast®	1.2 ± 0.1	0.8 ± 0.1	2.2 ± 0.4	15.9 ± 0.8	20.2 ± 1.0	31.7 ± 1.9	12.8 ± 0.8	4.4 ± 0.5	0.7 ± 0.1	18.2 ± 2.1	87.9 ± 2.2
bm3 + Celluclast®	0.9 ± 0.1	0.5 ± 0.1	n.d	12.9 ± 1.1	13.6 ± 1.7	25.1 ± 2.3	15.3 ± 0.5	4.6 ± 0.4	0.9 ± 0.1	37.4 ± 5.7	96.9 ± 5.1

n.d. = not detected; HC = sum of hemicellulosic monosaccharides; CC = crystalline cellulose; Total = Sum of plant and fungal components

Comparison before and after digestion with Ustilago maydis and without/with addition of Celluclast®. Data without Celluclast® are the results of two independent fermentation experiments with independent fungal inoculums and 4 or 6 plants of B73 or bm3, respectively. The AVG ± SD is calculated from the resulting n = 8 and n = 12 for B73 and bm3, respectively. The addition of Celluclast® is shown as AVG ± SD of two technical replicates for each individual plant, i.e. n = 8 and n = 12 replicates for B73 and bm3, respectively. Bold values indicate statistically significant differences between the material determined by a two-tailed students t-Test at p-value < 0.05