Table 1.
Commercial antibodies used in this study
| Antibody | Clone | Manufacturer | Catalogue # | Assay | Dilution | RRID |
|---|---|---|---|---|---|---|
| anti-Akt | 40D4 | Cell Signaling Technology | 2920 | Western Blot | 1:2000 | AB_1147620 |
| anti-phospho-Akt (Ser473) | D9E | Cell Signaling Technology | 4060 | Western Blot | 1:2000 | AB_2315049 |
| anti-β-actin | W16197A | Biolegend | 664802 | Western Blot | 1:2000 | AB_2721349 |
| anti-phospho-Stat3 (Tyr705) | D3A7 | Cell Signaling B1:H36 Technology | 9145 | Western Blot | 1:1000 | AB_2491009 |
| anti-Stat3 | 124H6 | Cell Signaling Technology | 9139 | Western Blot | 1:1000 | AB_331757 |
| anti-Fyn | E3 | Santa Cruz Biotechnology | SC-365913 | Western Blot | 1:500 | AB_10842309 |
| anti-Lyn | C13F9 | Cell Signaling Technology | 2796 T | Western Blot | 1:1000 | AB_2138391 |
| anti-phospho-src-famly (Tyr418) | EP503Y | Abcam | Ab40660 | Western Blot | 1:1000 | AB_776106 |
| anti-Mcam | 281 | Novus Biologicals | NBP2-90721 | Western Blot | 1:1000 | unavailable |
| anti-MCAM | EPR3208 | Abcam | Ab75769 | Western Blot | 1:1000 | AB_2143375 |
| anti-phospho-Pten (Ser380/Thr382/383) | 44A7 | Cell Signaling Technology | 9549 | Western Blot | 1:1000 | AB_659891 |
| anti-Pten | A2B1 | Santa Cruz Biotechnology | SC-7974 | Western Blot | 1–500 | AB_628187 |
| anti-phospho-Fak (Ty925) | polyclonal | Cell Signaling Technology | 3284 | Western Blot | 1:1000 | AB_10831810 |
| anti-phospho-Fak (Tyr397 | D20B1 | Cell Signaling Technology | 8556 | Western Blot | 1:1000 | AB_10891442 |
| anti-Fak | polyclonal | Cell Signaling Technology | 3285 | Western Blot | 1:1000 | AB_2269034 |
| anti-phospho-Stat5 (Tyr694) | D47E7 | Cell Signaling Technology | 4322 | Western Blot | 1:400 | AB_10544692 |
| anti-Stat5 | ST5-8F7 | Thermo Fisher Scientific | 33–5900 | Western Blot | 1:200 | AB_2533129 |
| anti-Rabbit-IgG-AF680 | polyclonal | Thermo Fisher Scientific | A-21076 | Western Blot | 1:1000 | AB_2535736 |
| anti-Mouse-IgG-AF790 | polyclonal | Thermo Fisher Scientific | A11375 | Western Blot | 1:1000 | AB_2534146 |
| anti-Rat-IgG-H&LDyLight-800 | polyclonal | Thermo Fisher Scientific | SA5-10032 | Western Blot | 1:10000 | AB_2556612 |
| anti-Paxillin | Y113 | Abcam | Ab32084 | Western Blot | 1:5000 | AB_779033 |
| HA-Tag | 2-2.2.14 | Invitrogen | 26183 | Western Blot | 1:1000 | AB_10978021 |
| c-Myc | 9E10 | Invitrogen | MA1-980 | Western Blot | 1:1000 | AB_558470 |
| phospho-CK2 substrate | MultiMab | Cell Signaling Technology | 8738S | Western Blot | 1:1000 | AB_2797653 |
| anti-Sox10 | SD204-04 | Thermo Fisher Scientific | MA5-32398 | Immunocytochemistry | 1:50 | AB_2809676 |
| anti-Krt14 | LL002 | Thermo Fisher Scientific | MA5-11599 | Immunocyto-chemistry | 1:50 | AB_10982092 |
| anti-Krt8 | TROMA-1 | DSHB U of Iowa | Ab-531826 | Immunocyto-chemistry | 1:50 | AB_531826 |
| anti-Rabbit-IgG-AF568 | polyclonal | Thermo Fisher Scientific | A-11011 | Immunocyto-chemistry | 1:200 | AB_143157 |
| anti-Mouse-IgG-AF647 | polyclonal | Thermo Fisher Scientific | A28181 | Immunocyto-chemistry | 1:200 | AB_2536165 |
| anti-Rat-IgG-AF647 | polyclonal | Thermo Fisher Scientific | A-21247 | Immunocyto-chemistry | 1:200 | AB_141778 |
| anti-Mcam | ME9F1 | Biolegend | 313605 | FACS | 1:100 | AB_345299 |
| anti-CD326 | G8.8 | Thermo Fisher Scientific | 25-5791-80 | FACS | 1:160 | AB_1724047 |
| anti-Sca1 | D7 | Thermo Fisher Scientific | 17-5981-81 | FACS | 1:300 | AB_469486 |
| anti-Ncam1 | EPR21827 | Abcam | Ab220360 | FACS | 1:500 | AB_2927664 |
| rabbit anti-FLAG | polyclonal | Invitrogen | 740001 | Immuno-precipitation | 1:50 | AB_2610628 |