AI accelerated the data extraction and analysis of LOs. (A) A new deep neural network was developed to dynamically capture and track trajectories of organoids over time during culture based on the dataset of high-throughput organoid images. Copyright 2021, Elsevier Ltd. (B) Data-driven feature learning has the ability to classify, cluster and visualize 3D images, as well as rapidly quantitative phenotyping analysis of LOs. Copyright 2021, The Company of Biologists Ltd. (C) Data-driven cell segmentation-free machine learning recorded the phenotypic responses of dense cortical neurons to pharmacological inhibition of anti-apoptotic proteins. Copyright 2021, PLoS [44,190,191].