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Expression of vhs from the inducible CUP1 promoter inhibits colony formation. Strains W303 pYEX-BX (vector), W303 pYEX-BX vhs (vhs), and W303 pYEX-BX vhs1 (vhs1) were grown to saturation in YNBD, and then equal amounts of cells from each strain were spotted and streaked onto YNBD (glucose), YNBG (galactose), YNBR (raffinose), and YNBM (maltose) plates containing (+) or lacking (−) 0.5 mM copper sulfate (Cu). The plates were incubated at 30°C for 5 days.
