FIG. 4.

Carbon source-dependent variation in the vhs-induced phenotype in liquid cultures. Strains W303 pYEX-BX (vector), W303 pYEX-BX vhs (vhs), and W303 pYEX-BX vhs1 (vhs1) growing in YNBD (glucose) were pelleted, washed in water, and resuspended in YNB containing the indicated carbon sources, in the presence or absence of 0.5 mM CuSO₄. The OD₆₀₀ of the cultures was then monitored over time. Closed symbols, not induced; open symbols, induced. Circles, empty vector; squares, vhs expression vector; triangles, vhs1 expression vector.