Table 1.

Primer Sequences Used for Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR)

Target Gene	Forward Primer	Reverse Primer
PI3KCA	GAAGCACCTGAATAGGCAAGTCG	GAGCATCCATGAAATCTGGTCGC
AKT1	TGGACTACCTGCACTCGGAGAA	GTGCCGCAAAAGGTCTTCATGG
mTOR	AGCATCGGATGCTTAGGAGTGG	CAGCCAGTCATCTTTGGAGACC
GAPDH	TGCACCACCAACTGCTTAGC	GGCATGGACTGTGGTCATGAG

Abbreviations: PIK3CA, Phosphatidylinositol 3-kinase catalytic subunit alpha; AKT1, Protein kinase B; PKB, mTOR, Mammalian target of rapamycin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.