. 2001 Feb;75(3):1186–1194. doi: 10.1128/JVI.75.3.1186-1194.2001

TABLE 1.

Potential BoHV-4 ORFs and homologues to HHV-8 and HVS

BoHV-4 ORF	Strand	Position of^a:		Size (aa)	HHV-8		HVS		Description (Fig. 1 abbreviation)
BoHV-4 ORF	Strand	Start	Stop	Size (aa)	%ID^b	ORF	%ID^b	ORF	Description (Fig. 1 abbreviation)
Bo1	−	633	445	62
3	+	614	4480	1,288			36.73	3	Tegument protein or v-FGAM-synthase (BORFA1^c)
Bo2	+	4608	4811	67					BORFA2^c
Bo3	+	5286	5513	75
6	+	5943	9347	1,134	56.33	6	58.65	6	Single-stranded DNA binding protein (MDBP)
7	+	9352	11430	692	28.00	7	21.43	7	Transport protein
8	+	11420	14044	874	54.30	8	51.13	8	gB
9	+	14184	17201	1,005	62.96	9	62.49	9	DNA polymerase (DPOL)
10	+	17297	18577	426	22.74	10	28.78	10	BORFB1^c
Bo4	−	19345	18848	165	25.97				Short ORF of IE1 RNA
Bo5	−			285	27.85	K5			IE1 RNA
Bo5d	−	19918	19414	167					Long ORF of IE1 RNA, exon IV
Bo5c	−	20200	20015	62					Long ORF of IE1 RNA, exon III
Bo5b	−	20438	20288	51					Long ORF of IE1 RNA, exon II
Bo5a	−	20553	20538	5					Long ORF of IE1 RNA, exon I
Bo6	+	20495	20788	97
Bo7	−	21194	20843	116
B08	+	21519	21722	67					Overlapping with 1.7-kb late RNA
16	+	22971	23651	226	26.59	16	26.45	16	v-Bcl-2 homologue (BORFB2^c)
17	−	25261	23714	515	43.82	17	49.36	17	Capsid protein
18	+	25260	26078	272	47.84	18	50.79	18
19	−	27706	26030	558	47.22	19	50.00	19	Tegument protein
20	−	28220	27408	270	37.84	20	40.98	20
21	+	28204	29541	445	32.50	21	34.72	21	Thymidine kinase (TK)
22	+	29552	31675	707	32.47	22	36.18	22	Glycoprotein H (gH)
23	−	32874	31672	400	34.84	23	40.97	23
24	−	35110	32852	752	48.58	24	50.90	24
25	+	35100	39221	1,373	66.54	25	68.03	25	Major capsid protein (MCP)
26	+	39257	40171	304	54.60	26	60.07	26	Capsid protein
27	+	40185	40823	212	22.22	27	24.75	27
Bo9	+	40833	41132	99
29	−			682	60.65	29	61.73	29	Cleavage and/or packaging protein
29b	−	42295	41156	379		29b		29b	Cleavage and/or packaging protein, exon II
30	+	42308	42550	80	49.29	30	36.49	30
31	+	42484	43125	213	41.18	31	52.68	31
32	+	43071	44441	456	31.92	32	36.05	32	Viral DNA cleavage or packaging protein
33	+	44434	45432	332	36.48	33	50.76	33	Dispensable for viral growth of HSV-1
29a	−	46330	45422	303		29a		29a	Cleavage and/or packaging protein, exon 1
34	+	46329	47315	328	42.81	34	44.76	34
35	+	47287	47760	157	31.76	35	37.33	35
36	+	47648	48952	434	33.57	36	37.44	36	Kinase (PK)
37	+	48960	50429	489	45.98	37	52.08	37	Alkaline exonuclease
38	+	50381	50587	68	32.73	38	29.03	38
39	−	51763	50654	369	54.08	39	50.82	39	Glycoprotein M (gM)
40	+	51879	53252	457	27.58	40	33.64	40	Helicase-primase complex component
41	+	53365	53866	173	26.06	41	31.82	41	Helicase-primase complex component
42	−	54753	53878	291	36.59	42	43.56	42
43	−	56380	54530	616	53.28	43	59.18	43	Capsid protein
44	−	56328	58706	792	61.12	44	67.73	44	Helicase
45	−	59535	58810	241	28.96	45	33.19	45
46	−	60296	59535	253	57.31	46	61.51	46	Uracil-DNA-glycosidase
47	−	60736	60305	143	29.69	47	33.33	47	Glycoprotein L (gL)
48	−	62387	60843	514	28.61	48	29.34	48
49	−	63504	62605	299	31.86	49	24.49	49
50	+			552	26.08	50	28.92	50	R transactivator homologue
50a	+	62591	62593	1					IE2, R transactivator homologue, exon I
50b	+	63529	65184	551					IE2, R transactivator homologue, exon II
Bo10	+			237					Glycoprotein gp80
Bo10a	+	65714	66242	176					Glycoprotein gp80, exon I (BORFD1^c)
Bo10b	+	66325	66617	97					Glycoprotein gp80, exon II (BORFD1^c)
52	−	67029	66643	128	39.02	52	38.05	52
53	−	67367	67095	90	36.78	53	32.56	53
54	+	67436	68284	282	32.86	54	35.97	54	dUPTase
55	−	68945	68343	200	46.70	55	51.30	55
56	+	68909	71440	843	46.03	56	48.79	56	DNA replication protein
57	+			420	32.23	57	32.42	57
57a	+	71534	71561	9		57		57	Posttranscriptional transactivator? exon I
57b	+	71651	72888	411		57		57	Posttranscriptional transactivator? exon II
58	−	74364	73312	350	29.27	58	27.53	58
59	−	75553	74378	391	42.97	59	37.19	59	DNA replication protein
60	−	76623	75706	305	63.93	60	66.23	60	Ribonucleotide reductase (RR), small subunit
61	−	79038	76657	793	53.91	61	58.25	61	Ribonucleotide reductase (RR), large subunit
62	−	80039	79020	339		62		62	Assembly or DNA maturation protein
63	+	79996	82815	939	33.30	63	35.06	63	Tegument protein
64	+	82830	90539	2,569	33.02	64	35.44	64	Tegument protein
65	−	90947	90555	130	40.83	65	44.88	65	Capsid protein
66	−	92218	90944	424	36.98	66	39.08	66
67	−	92928	92158	256	47.24	67	58.12	67	Tegument protein
67.5	−	93324	92932	130	39.74	67.5	55.29	67.5
68	+	93206	94555	449	46.43	68	48.85	68	Probable glycoprotein
69	+	94563	95456	297	48.00	69	52.53	69
Bo11				57					1.1-kb late RNA
Bo11b	−	96220	96209	3					Long ORF of spliced 1.1-kb late RNA, exon II
Bo11a	−	97218	97057	54					Long ORF of spliced 1.1-kb late RNA, exon I
Bo12	+	97972	98175	67
Bo13	+	99393	99617	74
71	−	99431	98883	182	27.41	K13/71	23.42	71	v-FLIP (BORFE2^c)
73	−	100337	99576	253	15.00	73	24.79	73	BORFE3^c
75	−	104745	100828	1,305	25.29	75	38.85	75	Tegument protein or v-FGAM-synthetase
Bo14	−	105340	104828	170					Proline rich (BORFF1^c)
Bo15	−	106592	106278	104
Bo16	+	106779	107048	89					BORFF2^c
Bo17	+	107313	108635	440					v-β-1, 6GnT (BORFF3-4^c)

Positions of the respective ORFs of BoHV-4 are given from the first nucleotide of the start codon ATG to the last nucleotide of the stop codon.

%ID, percent identity of the HHV-8 and HVS ORFs to the indicated BHV-4 ORF was calculated with the GAP program of the GCG package.

ORF designations as used by Lomonte et al. (27).