Fig. 1.

A single bout of exhaustive exercise induces transient cardiac glycogen accumulation. A. Schematic of the tissue collection timeline post-exercise. B. Cardiac glycogen normalized to protein and expressed relative to the rest group. n = 7–8 mice/group. C. Cardiac phosphorylated glycogen synthase (pGS, Ser641), normalized to coomassie stain intensity and expressed relative to the rest group. n = 7–8 mice/group. D. Cardiac total glycogen synthase (GS) protein content normalized to coomassie stain intensity and expressed relative to the rest group. n = 7–8 mice/group. E. Ratio of phosphorylated glycogen synthase to total glycogen synthase. n = 7–8 mice/group. F. Cardiac phosphorylated glycogen phosphorylase (pGP, Ser14) normalized to coomassie stain intensity and expressed relative to the rest group. n = 7–8 mice/group. G. Cardiac total glycogen phosphorylase (GP) normalized to coomassie stain intensity and expressed relative to the rest group. n = 7–8 mice/group. H. Ratio of phosphorylated glycogen phosphorylase to total glycogen phosphorylase. Data presented as mean ± SEM, ^ap<0.05 rel to rest, ^bp < 0.05 rel to 0hr, ^cp < 0.05 rel to 2hr and ^dp < 0.05 rel to 4hr.