Fig. 1.

Properties of photosensitizer MPPα and killing mechanism of PDT. (A) Phagocytic activity of OS cells on the photosensitizer MPPα as measured by CLSM. Scale bar: 50 μm. (B) Fluorescence quantitative analysis of OS cells by flow cytometry. (C) Fluorescence co-localization of organelle probes with photosensitizer MPPα as measured by CLSM. Scale bar: 50 μm. (D) Effect of MPPα-PDT on OS cells activity was tested using the CCK-8 assay. (E) The singlet oxygen fluorescent images of OS cells after MPPα-PDT treatment detected by SOSG. Scale bar: 50 μm. (F–I) DCFH-DA fluorescence images and flow quantitative analysis of OS cells after MPPα-PDT treatment. Scale bar: 100 μm. Analyses were repeated in triplicate (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.