Fig. 2.
The effect of MPPα-PDT on promoting apoptosis and ferroptosis. (A) The mitochondrial fragmentation of OS cells as observed by CLSM after MPPα-PDT. Scale bar: 30 μm. (B) The apoptosis rate of OS cells after MPPα-PDT treatment by flow cytometry analysis. (C) The ΔΨm of OS cells after MPPα-PDT treatment by flow cytometry analysis. (D) The expression of mitochondrial apoptotic proteins was detected by Western blot analysis after MPPα-PDT treatment. (E) Fluorescent images and flow cytometry analysis of lipid peroxides in OS cells. Scale bar: 100 μm. (F) Fluorescent images and flow cytometry analysis of lipid-ROS in OS cells. Scale bar: 100 μm. (G) Fluorescent images and flow cytometry analysis of Fe2+ in OS cells. Scale bar: 100 μm. (H) MDA contents in OS cells after various treatments. (I) The expression of ferroptosis related proteins in OS cells was detected by Western blot analysis. Analyses were repeated in triplicate (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.