Fig. 4.
Ferrintinophagy is inhibited by MPPα-PDT. (A–B) Fluorescence images of ADV-eGFP-LC3 in OS cells after various treatments. The white arrow indicates LC3 fluorescence within the nucleus. Scale bar: 50 μm. (C) The expression of autophagy related proteins after various treatments was detected by Western blot analysis. (D) The expression of Atg5 protein after siRNA treated was detected by Western blot analysis. (E) Effect of silencing Atg5 on OS cells activity was tested using CCK-8 assay. (F–G) Analysis of Fe2+ by flow cytometry after different treatments. (H) MDA contents in OS cells after various treatments. (I) mRNA expression of NCOA4, FTH1, FTL after different treatments as measured by qPCR. Analyses were repeated in triplicate (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.