Fig. 5.

Targeting NCOA4 enhances ferroptosis mediated by MPPα-PDT. (A) The impact of NCOA4 on the clinical prognosis of OS patients was analyzed using data from GSE42352 database. (B) Expression of NCOA4 in human OS and adjacent tissues was detected using IHC. (C) Basic expression of NCOA4 protein in OS cells was tested by Western blot analysis. (D) Expression of the protein in OS cells was tested by Western blot analysis after NCOA4 overexpression. (E–F) Analysis of LPO, Lipid-ROS and Fe²⁺ by flow cytometry after different treatments. (G–H) The expression of protein NCOA4, FTH1, FTL in OS cells after various treatments were detected by Western blot analysis. (I) Detection of iron ion deposition images in OS cells after different treatments using Prussian blue staining. Scale bar: 50 μm. (J) Observation of mitochondria in OS cells by Transmission Electron Microscopy. The red or blue arrow refers to the mitochondria in OS cells. Scale bar: 1 μm. Analyses were repeated in triplicate (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)