Fig. 7.

HERC1 interacts with NCOA4. (A) The expression of NCOA4 protein in OS cells after various treatments was detected by Western blot analysis. (B) co-IP images of NCOA4 protein and ubiquitin protein in OS cells. (C) co-IP images of NCOA4 protein and HERC1 protein in OS cells. (D–G) Images and analysis of immunofluorescence double staining in OS cells after MPPα-PDT treatment. Scale bar: 50 μm. (H) The expression of HERC1 in OS cells was detected by Western blot analysis. (I) Quantitative analysis of HERC1 protein expression in OS cells, with the value of GAPDH as the loading control. (J–K) The expression of proteins in HOS cells after various treatments was detected by Western blot analysis. (L–M) The expression of proteins in 143B cells after various treatments was detected by Western blot analysis. (N) co-IP images of NCOA4 protein and ubiquitin protein in OS cells. Analyses were repeated in triplicate (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.