Fig. 8.
Targeting HERC1 enhances the anti-tumor efficiency of MPPα-PDT. (A) The ΔΨm of OS cells after various treatments by flow cytometry analysis. (B) The apoptosis rate of OS cells after various treatments by flow cytometry analysis. (C) JC1 fluorescence images of OS cells after different treatments. Scale bar: 100 μm. (D) The expression of pro-apoptotic proteins after HERC1 knockout combined with MPPα-PDT was detected by Western blot analysis. (E–F) Detection of Fe2+ and ROS in OS cells after different treatments using flow cytometry. (G–H) Quantitative analysis of flow cytometry data. (I–J) Detection of lipid-ROS and LPO in OS cells after different treatments using flow cytometry. (K–L) Quantitative analysis of flow cytometry data. Analyses were repeated in triplicate (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.