Fig. 1.

A: Effect of SCD4 deficiency on body weight in mice. WT and SCD4^−/− mice were fed chow or a high-fat diet (HFD). During the 8-week experiment, body weight was monitored weekly. B: Adiposity in mice, calculated as the ratio of visceral white adipose tissue (vWAT) weight to body weight, expressed as a percentage. C: Ratio of heart weight to body weight. D: Glucose tolerance in mice was analyzed at the end of the experiment using an intraperitoneal glucose tolerance test. E: Effect of SCD4 deficiency on the expression of genes that are related to heart dysfunction. mRNA levels of α and β myosin heavy chain (Myh6 and Myh7), atrium natriuretic peptide (Nppa), and B-type natriuretic peptide (Nppb) were analyzed using quantitative real-time PCR and the 2^-ΔΔCt method. The data are expressed as the mean ± SD. n = 10–12 mice/group. ^ap < 0.05, versus WT chow; ^bp < 0.05, versus WT HFD; ^cp < 0.05, versus SCD4^−/− chow.