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. 2024 Jul 31;65(9):100612. doi: 10.1016/j.jlr.2024.100612

Fig. 3.

Fig. 3

Effect of SCD4 deficiency on myocardial lipid accumulation. A: Representative thin-layer chromatography plate of separated neutral lipids. Lipids were isolated according to the method of Bligh and Dyer (1959). B: After soaking in a mixture of 10% cupric sulfate and 8% phosphoric acid, the plate was burned in at 140°C, and lipids were quantified by densitometry. C: Triglyceride (TG) content was analyzed using gas chromatography-mass spectrometry. D: The number of lipid droplets (LDs) and E: LD size distribution were determined using transmission electron microscopy images at 6,000× magnification. F: Mean lipid droplet size measured on TEM images at 6,000× magnification (n > 250). The data are expressed as the mean ± SD. n = 10–12 mice/group. ap < 0.05, versus WT chow; bp < 0.05, versus. WT HFD; cp < 0.05, versus. SCD4−/− chow. HFD, high-fat diet.