Fig. 5.

Effect of Scd4 downregulation in HL-1 cells and primary neonatal cardiomyocytes on lipid accumulation. A: Scd1, Scd2, and Scd4 mRNA levels in HL-1 cells after Scd4 silencing. B: Level of accumulated lipids, based on Oil Red O dye absorption in HL-1 cells that were treated with stearic acid (C18:0). C: Representative thin-layer chromatographic plate of separated neutral lipids from HL-1 cells and D: results of the plate densitometric analysis. E: Representative images of lipid droplets (LDs) staining using BODIPY in primary SCD4-deficient cardiomyocytes. Representative images at 60× magnification are presented. Scale bar = 10 μm. F: The number of LDs and G: LDs size measured in primary neonatal SCD4-defcient cardiomyocytes treated with stearic acid (C18:0). The data are expressed as the mean ± SD. (A–D) n = 3 independent experiments. ^ap < 0.05, versus non-targ vehicle; ^bp < 0.05, versus non-targ 18:0; ^cp < 0.05, versus siSCD4 vehicle. (E-G) n = 3 independent cardiomyocytes isolation. At least 20 cells from each isolation in each experimental condition were analyzed. ^ap < 0.05, versus WT control; ^bp < 0.05, versus WT 18:0; ^cp < 0.05, versus SCD4−/− control.