Fig. 7.

Analysis of cardiac mitochondria by transmission electron microscopy. A: Representative images of mitochondria (yellow) and lipid droplets (red arrows) in the left ventricle. Representative images at 150,00× magnification are presented. Scale bar = 2 μm. B: Mitochondrial density (n > 600), C: average area of a single mitochondrion, and D: percentage of tissue area occupied by mitochondria, presented as a percentage of tissue (n > 250). The data are expressed as the mean ± SD. n = 3 mice/group. ^ap < 0.05, versus. WT chow; ^bp < 0.05, versus WT HFD; ^cp < 0.05, versus. SCD4^−/− chow. HFD, high-fat diet.