Fig. 8.

Impact of SCD4 deficiency on mitochondrial NADH dehydrogenase activity and reactive oxygen species (ROS) production. A: NADH dehydrogenase activity and representative images of NADH dehydrogenase activity-dependent staining of the mouse left ventricle. Cryosections (10 μm thick) were stained with NADH and nitro blue tetrazolium. The stained sections were observed with a 20× magnification objective. Scale bar = 40 μm. B: Reactive oxygen species level and representative images of mouse left ventricle staining with ROS-sensitive dye. Images were captured at 10× magnification. Scale bar = 80 μm. C: Ndufv2 mRNA levels in the heart were analyzed using quantitative real-time PCR and the 2^-ΔΔCt method. D: NADH dehydrogenase subunit B8 protein level in the left ventricle of the mouse heart. The data are expressed as the mean ± SD. n = 3 mice/group in A and B. n = 10–12 mice/group in C and D. ^aP < 0.05, versus WT chow; ^bp < 0.05, versus. WT HFD; ^cp < 0.05, versus SCD4^−/− chow. HFD, high-fat diet.