|
| Antibodies |
|
|
|
| Mouse α-pericardin |
DSHB |
RRID: AB_528431 |
| Rat α-actinin |
Gift from J.Saide |
N/A |
| Rabbit α-CRTC |
Gift from M.Montminy |
N/A |
| Sheep α-CRTC (Vertebrate) |
MRC PPU Reagents and Services |
#S279D |
| Mouse α-FLAG |
Sigma-Aldrich |
RRID: AB_259529 |
| α-H15/Nmr1 |
Gift from J. Skeath |
N/A |
| Mouse α-dystroglican |
Gift from A.Wodarz |
N/A |
| α- Zfh1 |
Gift from E.Olson |
N/A |
| α- DMef2 |
Gift from E.Olson |
N/A |
| α-Svp |
Gift from R.Cripps |
N/A |
| Alexa Fluor 488 donkey anti-sheep |
Invitrogen |
#A-11015; RRID: AB_2534082 |
| Alexa Fluor 488 goat anti-mouse |
Invitrogen |
#A-11001; RRID: AB_2534069 |
| Alexa Fluor 647 donkey anti-rabbit |
Invitrogen |
#A-31573; RRID: AB_2536183 |
| Alexa Fluor 594 phalloidin |
Invitrogen |
#A-12381; RRID: AB_2315633 |
| Alexa Fluor 647 goat anti-guinea pig |
Invitrogen |
#A-21450; RRID: AB_2535867 |
| Alexa Fluor 594 donkey anti-rabbit |
Invitrogen |
#A-21207; RRID: AB_141637 |
| MF20 (Myosin) |
Developmental Studies Hybridoma Bank |
Antibody Registry ID: AB_2147781 |
| AlexaFluor488 donkey anti-chicken |
Invitrogen |
#A-78948; RRID: AB_2921070 |
| AlexaFluor488 donkey anti-rabbit |
Invitrogen |
#A-21206; RRID: AB_2535792 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Trizol |
Invitrogen |
#15596026 |
| mTeSR-1 media |
StemCell Technologies |
#85850 |
| Thiazovivin |
StemCell Technologies |
#100–0247 |
| CHIR99021 (GSK-3β and GSK-3α inhibitor) |
Selleck Chemicals |
#1263 |
| Wnt-C59 (WNT pathway inhibitor) |
Selleck Chemicals |
#S7037 |
| TrypLE Express |
ThermoFisher Scientific |
#12604013 |
| RPMI |
ThermoFisher Scientific |
#11875093 |
| Recombinant Human Insulin |
ThermoFisher Scientific |
#RP-10935 |
| L-ascorbic acid |
Sigma-Aldrich |
#A92902 |
| L-carnitine |
Sigma-Aldrich |
#8.40092 |
| AlbuMAX Lipid-Rich BSA |
ThermoFisher Scientific |
#11020021 |
| Sodium-DL-Lactate |
Sigma-Aldrich |
#041529.K2 |
| vitamin A |
Life Technologies |
#1257010 |
| lipofectamine RNAi Max |
ThermoFisher Scientific |
#13778150 |
| Tyrode’s solution |
Sigma-Aldrich |
#T1788 |
| voltage sensitive dye Vf2.1 Cl, Fluovolt |
ThermoFisher Scientific |
#F10488
|
| Pluronic F127 |
ThermoFisher Scientific |
#P6867 |
|
CRTC in situ hybridization probe |
Molecular Instrument |
N/A |
|
CG9297 in situ hybridization probe |
Molecular Instrument |
N/A |
|
MHC in situ hybridization probe |
Molecular Instrument |
N/A |
|
Argk in situ hybridization probe |
Molecular Instrument |
N/A |
|
| Critical commercial assays |
|
|
|
| Gibson Assembly Cloning Kit |
New England BioLabs |
#E5510S |
| miRNAeasy Mini Kit |
Qiagen |
#217084 |
| QuantiTect Reverse Transcription Kit |
Qiagen |
#205311 |
| FastStart Essential DNA Green Master kit |
Sigma-Aldrich Corp. |
#06402712001 |
| SuperScript™ III Reverse Transcriptase |
Invitrogen |
#18080093 |
| RNAscope™ ISH Kit |
ACD Bio |
#322786 |
| NEBNext® Poly(A) mRNA Magnetic Isolation Module |
New England Biolabs |
NEB#E3370 |
| NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina®
|
New England Biolabs |
NEB #E7765 |
| Bradford Protein assay Kit |
Bio-Rad Laboratories |
#5000001 |
| Zymo Research Quick-RNA MircoPrep Kit |
Zymo Research |
#R1051 |
| QuantiTect Reverse Transcription kit |
Qiagen |
#205314 |
| iTaq SYBR Green |
Life Technologies |
#1725120 |
|
| Deposited data |
|
|
|
| R script for in silico TFBS analysis |
https://github.com/gvogler/Dondi_2024
|
N/A |
| RNA-Seq dataset |
GEO - Gene Expression Omnibus |
GEO: GSE271481
|
|
| Experimental models: Cell lines |
|
|
|
| Id1 overexpressing cell line |
Gift from the Wu lab |
derived from Burridge PW et al., 201553
|
|
| Experimental models: Organisms/strains |
|
|
|
|
D. melanogaster: CRTC[25–3]
|
Gift from M.Montminy |
N/A |
|
D. melanogaster: UAS-CRTC
|
Gift from M.Montminy |
N/A |
|
D. melanogaster: y, CREBb[Δ400]/FM7c
|
Gift from J. Thomas |
N/A |
|
D. melanogaster: CRTC RNAi (P{KK106414} VIE-260B) |
Vienna Drosophila Stock Center |
#100974 |
|
D. melanogaster: Pp2B RNAi (P{KK107714} VIE-260B) |
Vienna Drosophila Stock Center |
#103144 |
|
D. melanogaster: CRTC-Flag- (PBac{fTRG01044.sfGFP-TVPTBF}VK00033) |
Vienna Drosophila Stock Center |
#318324 |
|
D. melanogaster: NFAT RNAi (P{KK102385} VIE-260B) |
Vienna Drosophila Stock Center |
#107032 |
|
D. melanogaster: CG9297 (tmn) RNAi (w1118; P{GD5649} v43222) |
Vienna Drosophila Stock Center |
#43222 |
|
D. melanogaster: KK background control |
Vienna Drosophila Stock Center |
#60100 |
|
D. melanogaster: GD background control |
Vienna Drosophila Stock Center |
#60000 |
|
D. melanogaster: tinCΔ4-GaI4 |
Lo, P.C., and Frasch, M., 2001 |
N/A |
|
D. melanogaster: Hand4.2-Gal4 |
Han and Olson, 2005, Dev54
|
N/A |
|
D. melanogaster: Dot-Gal4 (P{Ugt36A1-GAL4.K}43A, y1 w*) |
Bloomington Drosophila Stock Center |
#6903 |
|
D. melanogaster: sns-Gal4 (y1 w*; Mi{Trojan-GAL4.1}snsMI03001-TG4.1) |
Bloomington Drosophila Stock Center |
#76160 |
|
D. melanogaster: elav-Gal4 (P{GAL4-elav.L}2/CyO) |
Bloomington Drosophila Stock Center |
#8765 |
|
D. melanogaster: CG-Gal4 (y1 sc* v1 sev21; P{TRiP.HMC05950}attP40) |
Bloomington Drosophila Stock Center |
#65147 |
|
D. melanogaster: Lsp2-Gal4 (y1
w1118; P{Lsp2-GAL4.H}3) |
Bloomington Drosophila Stock Center |
#6357 |
|
D. melanogaster: Mef2-Gal4 (w*; P{Mef2-GAL4.247}3) |
Bloomington Drosophila Stock Center |
#50742 |
|
D. melanogaster: CG9297 (tmn) OE |
This paper |
N/A |
|
D. melanogaster: Pp2B-14B OE |
Drosophila Genomic Resource Center |
#116254 |
|
D. melanogaster: y[1]w[67c23]
|
Bloomington Drosophila Stock Center |
#6599 |
|
D. melanogaster: w1118
|
Bloomington Drosophila Stock Center |
#3605 |
|
Danio rerio: Tg(myl7:nls-EGFP)
|
Boston Children’s Hospital (Burns group) |
Gonzalez-Rosa J.M. et al., 201855
|
|
| Oligonucleotides |
|
|
|
| CRTC3 (MO sequence: TCCTAATTTGGCTGAGCTTACCCTT) |
Gene Tools, LLC |
Manchenkov T. et al., 201544
|
|
D. melanogaster RP49 qRT-PCR primers 5’ AAACGCGGTTCTGCATGAG 3’ 3’GCCACCAGTCGGATCGATAT 3’ |
IDT (Integrated DNA technologies) |
N/A |
|
D. melanogaster CRTC qRT-PCR primers 5’ CCCCTCCCTTTGATTGCCTA 3’ 3’ TCGGAAGTGTAAGGCCCATT 3’ |
IDT (Integrated DNA technologies) |
N/A |
|
D. melanogaster CG9297 qRT-PCR primers 5’ GGAAAAGCCAGCACCAGTTG 3’ 3’ GTCCCGCAGGATGATCTCAG 3’ |
IDT (Integrated DNA technologies) |
N/A |
|
Danio rerio Ef1a qRT-PCR primers 5’ AGAAGGCTGCCAAGACCAAG 3’ 3’ AGAGGTTGGGAAGAACACGC 5’ |
IDT (Integrated DNA technologies) |
N/A |
|
Danio rerio CRTC1 qRT-PCR primers 5’ AGCAGTACCAGCAACCTGAC3’ 3’ GAGTTGGCAGTTCCACCTGT 5’ |
IDT (Integrated DNA technologies) |
N/A |
|
Danio rerio CRTC2 qRT-PCR primers 5’ CTCGGGGAAATAGGACACGG 3’ 3’ CTGGGTAAGGCAACTTGGGT 5’ |
IDT (Integrated DNA technologies) |
N/A |
|
Danio rerio CRTC3 qRT-PCR primers 5’ CTCTCAGCTGCACTCGTCTC 3’ 3’ CTCCATTGGCAAGTTGCTGG 5’ |
IDT (Integrated DNA technologies) |
N/A |
|
hIPSC-CMs hGAPDH qRT-PCR primers 5’ GGAGCGAGATCCCTCCAAAAT 3’ 3’ GGCTGTTGTCATACTTCTCATGG 5’ |
Harvard Primer Bank |
N/A |
|
hIPSC-CMs hCRTC1 qRT-PCR primers 5’ TCAGTGGACAAACACGGACG 3’ 3’ CAGGGCGGAGTCAGAATTG 5’ |
Harvard Primer Bank |
N/A |
|
hIPSC-CMs hCRTC2 qRT-PCR primers 5’ GGGGCAGTTGTTTCGACTACC 3’ 3’ GGACTGGGGTTCATCACACTT 5’ |
Harvard Primer Bank |
N/A |
|
hIPSC-CMs hCRTC3 qRT-PCR primers 5’ GCAACTGCGCCTTACACAGTA 3’ 3’ TGAAATGACGGCTGAAACTCTG 5’ |
Harvard Primer Bank |
N/A |
|
hIPSC-CMs hSrl qRT-PCR primers 5’ GAAGAAGCCCCATTGAGGGAC 3’ 3’ CACCGCAGAGTAGTCATCGG 5’ |
Harvard Primer Bank |
N/A |
|
CG9297 PCR primers (cloning): 5p: ATGGG GCGGCTAACCATTTG 3p: TCAACTCGTCCTCTTAGGCT |
Harvard Primer Bank |
N/A |
|
hIPSC siRNA targeting sequence control UGGUUUACAUGUCGACUAAUGGUUUACAUGUUGUGUGAUGGUUUACAUGUUUUCUGAUGGUUUACAUGUUUUCCUA |
Dharmacon- ON TARGET plus |
D-001810–10-20 |
|
hIPSC siRNA targeting sequence hCRTC1 GCGGAAAUUCAGCGAGAAGGCACAGAGGCAAGGCGUACAGGACAAACACGGACGGCAGAGUUCAACAUGAUGGAGAA |
Dharmacon- ON TARGET plus |
L-014026–01-0005 |
|
hIPSC siRNA targeting sequence hCRTC2 GGCUAAACAUGAGUGAGCAAAUUUAGUGAGAAGAUGCUCAGGGCCUAACAUCAUCAGGCAGGCAGUCUCAUUA |
Dharmacon- ON TARGET plus |
L-018947–00-0005 |
|
hIPSC siRNA targeting sequence hCRTC3 CGUCAGAGUUUCAGCCGUC CUUCAGCAACUGCGCCUUA GAAGAGACGUUUCGAGCUG GCGACAAUUUGAUGGUAGU |
Dharmacon- ON TARGET plus |
L-014210–01-0005 |
|
hIPSC siRNA targeting sequence hSrl AAAACAGGGUGUAGCGAAA |
Dharmacon- ON TARGET plus |
J-022618–19-0010 |
|
| Recombinant DNA |
|
|
CG9297 cDNA clone in pFLC-I vector |
DGRC |
#RE20510 |
| pUASTattB vector |
DGRC |
#EF362409
|
|
| Software and algorithms |
|
| ImageJ |
https://imagej.nih.gov/ij/
|
N/A |
| SOHA |
SOHAsoftware.com
|
N/A |
| STAR aligner |
https://code.google.com/p/rna-star/
|
N/A |
| Cufflinks Cuffdiff package |
https://github.com/cole-trapnell-lab/cufflinks
|
N/A |
|
| Other |
|
| Sequencing |
Plasmidsaurus |
N/A |
|
Primordium Labs |
|
