Fig. 2. Brominated phosphoinositide binding to TRPV1.

a, The chemical structure of brominated phosphoinositides used in this study. The part of the lipids with resolvable density is outlined with dashed gray lines. b,c, VBP bound with PI–Br₄ (b) and PIP₂–Br₄ (c). d,e, Density map of key functional groups for PI–Br₄ (d) and PIP₂–Br₄ (e) with corresponding difference maps. Difference maps were determined by subtracting calculated map of models lacking bromine atoms and the phosphate moieties at 4 and 5 positions of the inositol headgroup from the experimental map (Methods). Both positive (green) and negative (orange) difference densities are shown.