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. 2024 Apr 24;31(9):1341–1354. doi: 10.1038/s41594-024-01292-9

Extended Data Fig. 8. Analysis of the heterogenous occupancy of LincGET-speckles and PCBP1 around the Carm1 gene loci.

Extended Data Fig. 8

a. Illustration of the analysis of the heterogenous occupancy of LincGET-speckles and PCBP1 around the Carm1 gene loci using immunofluorescence (IF) combined with RNA-FISH and DNA-FISH. Representative fluorescence images are shown which exhibit the occupancy of LincGET-speckles or PCBP1 around the Carm1 gene loci in late two- and early four-cell embryos. Scale bar, 10 μm. DNA & F-actin, the nucleus and membrane are displayed together with SYTOX™ Deep Red Nucleic Acid Stain (Invitrogen, S11380) and Alexa Fluor™ 647 Phalloidin (Invitrogen, A22287) to show the three-dimension structure of embryos. b, c. The dot plots showing the counting results of the number (NO.) of LincGET-occupied or PCBP1-occupied Carm1 gene loci in late two- (b) and early four-cell embryos (c). To prevent the dots from overlapping completely, the y-axis is divided into three groups with NO. = 0, 1, and 2, respectively.

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