Extended Data Fig. 9. Analysis of the heterogeneity of exon 3 to 6 skipping events of Carm1 pre-mRNAs.
a. Illustration of the analysis of the heterogeneity of exon 3 to 6 skipping events of Carm1 pre-mRNAs via single-cell SPAR assay. b, c. Agarose gel analysis of single-cell SPAR assay showing the heterogeneity of exon-skipping events on exon 3 to 6 of Carm1 pre-mRNAs in late two-cell (b) and early four-cell (c) embryos. The percentages are shown in bar plots below the gel. L, DNA ladder. Note that the embryos #1 to #24 of both L2C and E4C are also used in the results for exon 3 to 6 skipping splicing in Extended Data Fig. 2b, but the spatial position information are shown here with different colors for pB and npB as well as nB and fB, but not in Extended Data Fig. 2b.