Fig. 1.

KI/R downregulates HSPA12A in kidneys and TEC. A HSPA12A expression in different tissues. The indicated tissues were collected from adult C57BL/6 mice. Immunoblotting for HSPA12A was performed. Blots for α-Tubulin served as loading controls. n = 4. B Characterization of HSPA12A in TEC of kidneys. Frozen sections of human kidney were prepared for immunostaining against HSPA12A (green). CK–18 was used to indicate TEC (red). DPAI was counter stained nuclei (blue). Scale bar = 20 μm. C Experimental setting of mice. D Effects of KI/R on HSPA12A expression in kidneys. Following KI/R or sham surgery, kidneys were collected to examine HSPA12A expression by immunoblotting. Blots for GAPDH served as loading controls. Data are mean ± SD, **P < 0.01 by Student’s two-tailed unpaired t-test. n = 5. E Experimental setting of primary TEC. F Effects of H/R on HSPA12A expression in TEC. Primary TEC were subjected to H/R followed by examination of HSPA12A expression by immunoblotting. Data are mean ± SD, **P < 0.01 by Student’s two-tailed unpaired t-test. n = 3