Fig. 3.

Distinct melanoma cell states identified in tumour biopsies. a) UMAP plot of aggregated (from all 20 samples) malignant melanoma cells showing four cell clusters, annotated as melanocytic, transitory, neural crest-like and undifferentiated. b) UMAP plot and c) heatmap showing the relative expression of the progressive differentiation gene signatures⁶ in the four cell clusters. Scale bars shows relative high (red) vs low (blue) expression of the signatures. d) Proportion of melanoma cell states in individual tumour samples treated with DMSO control or DT. e) Changes in cell state proportions in matched DMSO control or DT-treated tumour dissociates. Orange symbols indicate NAIVE while green symbols indicate IO PROG patient samples. Data comparison between DMSO and DT, and NAIVE vs IO PROG performed using the linear mixed effect model; p values shown in Supplementary Table S4.