FIG. 7.

Kinetics of generation of NS5A proteins. (A) 9-13 cells were seeded 60 h prior to radiolabeling with [³⁵S]methionine-cysteine (200 μCi/ml) for 10 min followed by incubation with nonradioactive medium for the times given above the lanes. Cells were lysed, and NS5A proteins were analyzed by immunoprecipitation, SDS-PAGE, and autoradiography. (B) Quantification of NS5A-specific bands by phosphorimaging. Values obtained at time point zero were set at 100%.