TABLE 1.
Summary of reproductive statistics estimated for juvenile red swamp crayfish (Procambarus clarkii) for four sites (East Golf Course 2, East Golf Course 4, Meadowbrook 1, and Hotel1).
| EastGC2 | EastGC4 | WestGC1 | Hotel1 (cohort 1) | Hotel1 (cohort 2) | Hotel1 (combined) | ||
|---|---|---|---|---|---|---|---|
| Cohort dates | Fall2020–Spring2021 | Fall2020–Spring2021 | Fall2020–Spring2021 | Fall2020–Spring2021 | Summer2021–Fall2021 | Fall2020–Fall2021 | |
| Sample size | 118 | 294 | 218 a | 90 | 147 | 237 a | |
| N b_LD | 172.2 (105.3–390.7) | 197.3 (154.7–261.8) | 66.7 (44.8–105.3) | 27.0 (20.1–37.3) | 17.6 (15.3–20.3) | 33.7 (29.3–38.8) | |
| N b_Wang | 145 (114–191) | 253 (211–302) | 121 (96–157) | 33 (21–55) | 24 (14–43) | 49 (34–73) | |
| N s | 113 | 230 | 152 | 46 | 41 | 81 | |
|
|
124.9 ± 5.6 | 243.2 ± 5.5 | 169.9 ± 7.4 | 74.6 ± 19.6 | 45.1 ± 4.0 | 93.0 ± 7.6 | |
| RS (95% CI) | 1.62 ± 0.055 | 1.32 ± 0.053 | 1.62 ± 0.085 | 3.50 ± 0.148 | 4.25 ± 0.273 | 2.58 ± 0.155 | |
| (1.52–1.74) | (1.23–1.44) | (1.47–1.81) | (3.26–3.85) | (3.75–4.84) | (2.31–2.94) | ||
| Θ | 0.004 | 0.002 | 0.004 | 0.015 | 0.021 | 0.010 |
Note: Definition of cohorts by collection dates, sample sizes of red swamp crayfish juveniles used in analyses, and the estimated effective number of breeders estimated using NeEstimator (N b_LD) and Colony (N b_Wang). N b_LD estimates are followed by a jackknife confidence interval and N b_Wang estimates are followed by 95% confidence intervals. Estimates of reconstructed parental genotypes based on the pedigrees without adjustments (N s) and estimates of the asymptotic number () of adults based on the Chao estimate with a standard error, estimated mean rarefied reproductive success (RS) of contributing adults with aSD and 95% confidence interval, and mean offspring coancestry (Θ) are also included. Analyses are based on 930 genetic markers (single nucleotide polymorphisms, SNPs), except for N b_LD which was based on 2262 SNPs.
The total number of samples genotyped was 249 for WGC1 and 241 for Hotel1, but samples were removed (WGC1 N = 30 and Hotel1 N = 4) because they had a carapace length >20 mm or were in a second cohort collected from WGC1 that did not have a large enough sample size to analyze.