Fig. 1.

Endometrial stem cell isolation and characterization. A) Phase contrast photomicrograph of human endometrial stem cells cultured in the third passage and differentiated cells after 21days of induction into osteoblast and adipocyte cells. The cells usually appeared elongated and spindle-shaped with round nuclei. Scale bare: 100 μm.Microphotographs of isolated EnSCs after 21 days culture in osteogenic and adipogenic induction media stained with alizarin red and oil red o staining. (scale bar is 50 μm). B) Flow cytometric analysis of isolated EnSCs for mesenchymal stem cell markers (CD90, CD105 and CD146), hematopoietic marker (CD34), endothelial marker (CD31). The isolated cells are positive for CD90, CD105 and CD146 and are negative for CD31, CD34. C) Detailed analysis of cultured EnSCs showed normal karyotypes containing 23 pairs of chromosomes.