Table-1.
Primers used for conventional and real time RT-PCR.
| Genes | Primer sequence (5″–3″) | Size (pb) | Annealing (◦C) |
|---|---|---|---|
| IL-10 (Rat) | F:AGAGAACCATGGCCCAGAAA R:GGGAGAAATCGATGACAGCG |
99 bp | 59 |
| IL-6 (Rat) | F:GCAAGAGACTTCCAGCCAGT R: CTGTGAAGTCTCCTCTCCGG |
140 bp | 59 |
| TGF-B (Rat) | F: CTTCCCCTCCGAAACTGTCT R: ATGGCATCAAGGTACCCACA |
105 bp | 60 |
| HSP70 (Rat) | F: CTCCGCTTCTCTGCTTCTCT R: CTCGCTGATTGGCCCATG |
119 bp | 60 |
| Gapdh (Rat) | F: GTTGTGGATCTGACATGCCG R: CCTCAGTGTAGCCCAGGATG |
102 bp | 59 |
| Gapdh (Human) | F: TGGTATCGTGGAAGGACTCA R: CCAGTAGAGGCAGGGATGAT |
132 bp | 60 |