Fig. 4.
Theoretical Monte Carlo simulations of single-molecule FRET efficiencies of a six-site migrating HJ. The binning time is 6 ms. The central efficiency at each of the six sites is shown as a heavy line on the rightmost axis. (a) The FRET efficiency for long residence time (slow hopping), τR = 10 tbin. The segmented line is the same data without Gaussian noise. (b) Same as a but for a short residence time (rapid hopping), τR = 1/20 tbin. The error bar on the right shows the time-averaged efficiency 〈E〉t and its deviation δE.