Figure A4.

(A) Immunofluorescence of Sox9, Cpa1 and Krt19 (top panel) and Cpa1, Hes1 and Krt19 (bottom panel) at given time points following caerulein treatment of young mice (n = 3). Images were acquired using confocal. (B) Immunofluorescence of Ecad, Krt19 and Mist1 at given timepoints after caerulein treatment of young mice. DAPI was used as a counterstain to visualise nuclei. Images were acquired using ScanR, and scale bars represent 50 μm. (C) Quantitative real-time PCR of markers associated with acinar plasticity at given time following caerulein treatment in young mice. Mist1 is acinar-specific, whereas Krt19 is expressed in mature ductal cells and ADM. Fold changes are shown relative to control and normalised with the housekeeping genes Rpl5 and Rps29. Statistical analysis was determined by unpaired one-way ANOVA. n = 3 mice per group. Error bars represent SD. Values of significance: ns P > .05 is omitted, ∗∗ P ≤ .01, ∗∗∗ P ≤ .001, ∗∗∗∗ P ≤ .0001. (D) Immunofluorescence of Mist1 in young control and following 4 injections with caerulein. Nuclei were counterstained by DAPI. Images were acquired using confocal microscopy, and scale bars represent 50 μm. Quantification of IF showing mean fluorescent intensity (MFI) of Mist1 in arbitrary units. Statistical analysis was determined by an unpaired t-test. n = 3 mice per group. Error bars represent SD. Values of significance: ns P > .05, ∗ P ≤ .05.