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(A) We examined the collagen deposition in the extracellular matrix by Fast Green and Sirius Red (FGSR) staining of young and aged mice at the indicated time points. Scale bars represent 250 μm. (B) Collagen deposition, visualized by Sirius Red stain, was quantified as percentage positive area of total tissue area. Statistical analysis was determined by two-way ANOVA. Error bars represent SD. Values of significance: ns P > .05 is omitted, ∗∗P ≤ .01, ∗∗∗∗ P ≤ .0001.
