Figure A7.

(A) Principal component analysis (PCA) of RNA-seq data reveal clustering of each condition, regardless of age. PCA score plot of PC1 and PC2 illustrates 87.42 % of the total variance in transcriptomic data between young control (red), young 5H (green), aged 5H (blue) and aged control (purple). PCA was done by Novogene. (B) Gene ontology analyses of differentially expressed genes in the young and aged control tissues. (C and D) GSEA of hallmark signatures gene sets between 5H and control in young mice (C) and aged versus young 5H (D). Significantly enriched hallmark signature gene sets were determined by FDR q-value < 0.25 and nominal P-value < .05. (E) Differentially expressed genes related to acinar plasticity and differentiation compared between the 5-hour timepoint and control for each age group. (F) Volcano plots showing statistical significance (−log10 P-value) on the y-axis versus magnitude of fold change (log2 FC) on the x-axis. Each dot represents a gene. The genes are colour-coded by adjusted P-value (padj) < .05 (blue) and > .05 (red).