Figure 2.

Aging does not affect the capacity of acinar cells to enter the cell cycle. (A) Immunofluorescence of Ecad and Ki67 at given time points after caerulein treatment in young and aged mice. DAPI was used as a counterstain to visualize nuclei. Images were acquired using ScanR, and representative images were shown (n = 3). Scale bars represent 50 μm. (B) Percentage of epithelial cells (Ecad+) that are proliferating (Ki67+). (C) Percentage of non-epithelial cells (Ecad-) that are proliferating (Ki67+). Statistical analysis was determined by two-way analysis of variance. Error bars represent SD. Values of significance: ns P > .05 is omitted, ∗P ≤ .05, ∗∗P ≤ .01, ∗∗∗P ≤ .001, ∗∗∗∗P ≤ .0001. DAPI, 4′,6-diamidino-2-phenylindole.