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. 2024 Aug 14;300(9):107672. doi: 10.1016/j.jbc.2024.107672

Figure 1.

Figure 1

ZMYND8 associates with USP7.A, HEK293T cells transfected with constructs encoding Flag tagged deubiquitinases (DUBs) were subjected to co-immunoprecipitation (co-IP) assays using the anti-Flag magnetic beads followed by western blotting using anti-ZMYND8 antibody. B and C, Co-IP assay of endogenous ZMYND8 with USP7 and vice versa in MDA-MB-231 cells or MDA-MB-468 cells. WCL, whole cell lysate. D and E, MDA-MB-231 cells (D) or MDA-MB-468 cells (E) were co-stained with anti-ZMYND8 and anti-USP7 antibodies using immunofluorescence staining to determine the colocalization of ZMYND8 and USP7. Nuclei were counterstained with DAPI. Scale bar: 25 μm.