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. 2024 Aug 14;300(9):107672. doi: 10.1016/j.jbc.2024.107672

Figure 3.

Figure 3

USP7 modulates ZMYND8 stability.A, protein levels of endogenous ZMYND8 were analyzed in MDA-MB-231 cells and MDA-MB-468 cells stably expressing wild type (WT) or catalytically inactive (C223S) Myc-USP7. B, protein levels of endogenous ZMYND8 in control or USP7-knockdown (USP7-KD) MDA-MB-231 cells and MDA-MB-468 cells. C, mRNA levels of ZMYND8 were analyzed by RT-qPCR in MDA-MB-231 cells and MDA-MB-468 cells stably WT or C223S Myc-USP7. ns, no significance, by 1-way ANOVA Tukey’s multiple comparisons test. D, mRNA levels of ZMYND8 were analyzed by RT-qPCR in MDA-MB-231 cells and MDA-MB-468 cells with USP7 ablation. ns, no significance, by 1-way ANOVA Tukey’s multiple comparisons test. E and F, the half-life of ZMYND8 protein in MDA-MB-231 (E) or MDA-MB-468 (F) cells expressing Myc-USP7. Cells were treated with 10 μg/ml CHX for the indicated time. G and H, the half-life of ZMYND protein were analyzed in scrambled shRNA (SC) or USP7-KD#3 MDA-MB-231 (G) or MDA-MB-468 (H) cells. Cells were treated with 10 μg/ml CHX for the indicated time.