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. 2024 Aug 14;300(9):107672. doi: 10.1016/j.jbc.2024.107672

Figure 5.

Figure 5

K1034 is the key deubiquitination site for ZMYND8 by USP7.A, ubiquitination of full length and truncated ZMYND8 in HEK293T cells treated with 10 μM MG132 for 6 h before harvest. B, ubiquitination of WT and mutant ZMYND8 in HEK293T cells treated with 10 μM MG132 for 6 h before harvest. C and D, ubiquitination of ectopic WT and K1034A mutant Flag-ZMYND8 in MDA-MB-231 (C) and MDA-MB-468 (D) cells treated with 10 μM MG132 for 6 h before harvest. E, ubiquitination of endogenous ZMYND8 in MDA-MB-231 cells stably expressing WT or K1034A mutant Flag-ZMYND8 together with Myc-FBXW7.