.USP7 expression is positively correlated with ZMYND8 expression in human breast tumors.A–D, protein levels of USP7, ZMYND8, VEGFA, and ZEB1 in human breast tumors (n = 7) and adjacent normal breast tissues (n = 7) (A, B and D) and the correlation of USP7 expression and ZMYND8 expression in human breast tumors and normal breast tissues (B). ∗p < 0.05, by two-tailed t test. P, patient. B, the correlation of USP7 expression and ZMYND8 expression in human breast tumors and normal breast tissues. E–G, Pearson correlation analysis of USP7 mRNA with ZEB1 and VEGFA mRNAs in human breast cancer tissues. The data were retrieved from the GSE115577 (E), GSE119295 (F), and GSE208102 (G) databases. H, Kaplan-Meier survival analysis for patients with breast cancer using datasets downloaded from the PrognoScan database. I, a proposed model of USP7-mediated ZMYND8 stability in breast cancer. USP7 directly interacts with ZMYND8 and antagonizes FBXW7-mediated ZMYND8 ubiquitination and degradation, thereby promoting breast cancer cell migration and invasion by stimulating the transcription of ZMYND8 target genes, such as VEGFA and ZEB1.