Impact of DAR on levels of (a) tissue concentration and
exon skipping
in (b) gastrocnemius, (c) quadriceps, (d) diaphragm, and (e) heart
muscles. mdx mice were treated with a single IV injection
of 10 mg/kg PMO dose for the DAR3.9 and DAR5.8 AOCs, and 12 mg/kg
for the DAR9.7 AOCs. Mice (n = 4 per group, error
bars represent SEM) were treated with different combinations of linkers
(MCC or MC), DARs, and PMO conjugated to αmTfR1. Control groups
included B10 WT mice and mdx mice treated with IV
PBS. Tissues were harvested at 14 days post-treatment and exon skipping
was assessed by ddPCR. Statistical analysis was performed using one-way
ANOVA, with all groups compared against αmTfR1-MCC-pmoEx23 DAR4.
*: P ≤ 0.05, **: P ≤
0.01, ***: P ≤ 0.001; ****: P ≤ 0.0001. ANOVA, analysis of variance; ddPCR, droplet digital
polymerase chain reaction; IV, intravenous; ns, not significant.