KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
| Anti-GFP | Life Technologies | Cat# A10262; RRID: AB_2534023 |
| Anti-NMDAR1 | Synaptic Systems | Cat# 114011; RRID: AB_887750 |
| Anti-AMPAR1 | Synaptic Systems | Cat# 182003; RRID: AB_2113441 |
| Anti-AMPAR2 | Synaptic Systems | Cat# 182103; RRID: AB_2113732 |
| Anti-Bassoon | Synaptic Systems | Cat# 141004; RRID: AB_2290619 |
| anti-Guinea pig-405 | AbCam | Cat # ab175678; RRID: AB_2827755 |
| anti-Chicken-488 | Invitrogen | Cat# A11039; RRID: AB_142924 |
| anti-Mouse-AF+555 | Invitrogen | Cat # A32727; RRID: AB_2633276 |
| anti-Rabbit-AF+647 | Invitrogen | Cat# A32733; RRID: AB_2633282 |
|
Bacterial and virus strains | ||
| AAV2-hSyn-hChR2(H134R)-mCherry | Gift from Karl Deisseroth | Addgene plasmid # 26976; RRID: Addgene_26976 |
| pENN.AAV.CamKII 0.4.Cre.SV40 | Gift from James M. Wilson | Addgene viral prep # 105558-AAV1; RRID: Addgene_105558 |
| pAAV-hSyn-DIO-EGFP | Gift from Bryan Roth | Addgene viral prep # 50457-AAV1; RRID: Addgene_50457 |
|
Chemicals, peptides, and recombinant proteins | ||
| TTX | Tocris | 1078 |
| 4-AP | Sigma-Aldrich | A78403 |
| MNI-caged-L-glutamate | Tocris | 1490 |
| Alexa 594 | Invitrogen | A10438 |
| Alexa 488 | Invitrogen | A10436 |
| OGB-1 | Thermo Fisher | O6806 |
| D-APV | Tocris | 0106 |
| DNQX disodium salt | Tocris | 2312 |
| DAPI | Thermo Fisher | 62248 |
| Picrotoxin | Tocris | 1128 |
| Acrylamide | MilliporeSigma | A9099 |
| Sodium acrylate | MilliporeSigma | 408220 |
| Bis-acrylamide | Bio-Rad Laboratories | 161–0142 |
| VA-044 | Wako Chemicals | NC0632395 |
| Sodium dodecyl sulfate | Sigma-Aldrich | L3771 |
| Sodium sulfite | Sigma-Aldrich | S0505 |
|
Experimental models: Organisms/strains | ||
| Mouse: C57BL/6 | Charles River | Cat# 027; RRID:IMSR_CRL:027 |
| Mouse: Thy-1-GFP-M | Jackson Labs | Cat# 007788; RRID:IMSR_JAX:007788 |
|
Software and algorithms | ||
| MATLAB (R2019a) | MATLAB | RRID:SCR_001622; |
| ImageJ (v1.54f) | National Institutes of Health | RRID:SCR_003070; https://imagej.nih.gov/ij/index.html |
| Custom code for data analysis | This paper | Zenodo: https://zenodo.org/doi/10.5281/zenodo.12791375 |
|
Other | ||
| Ultima In Vitro Multiphoton Microscope System | Bruker | RRID: SCR_017142 |
| MaiTai DeepSee | Spectra-Physics | MAI TAI HP DS |
| Electro-optical modulator | Conoptics | M350–50 |
| Photosensor module | Hamamatsu | H7422A-40 |
| Collimated LED for Olympus BX | ThorLabs | M625L4-C1 |
| 473 nm laser | OptoEngine LLC | MSL-FN-473/1~100mW |
| Dagan BVC-700A | Dagan Corporation | N/A |
| Fully automated vibrating blade microtome | Leica | Cat #VT1200S; RRID: SCR_018453 |
| TCS SP8 upright confocal microscope | Leica | DM6000 |
| Intracellular Microinjection Dispense System | Parker Hannifin | Picospritzer III; RRID: SCR_018152 |
| Flexible Stimulus Isolator Unit | A.M.P.I. | ISO-Flex; RRID: SCR_018945 |
| Thorlabs Bergamo® II Multiphoton | Thorlabs | N/A |
| Ti:Sapphire laser | Coherent | Chameleon Ultra II |