FIG. 6.
Generation of M gene mutants by site-directed mutagenesis. (A) Model of M protein topology with amino terminus-exo carboxy terminus-endo topology in the virion membrane. Predicted glycosylation sites are indicated by asterisks. Numbered arrows indicate the amino acid positions in the model. (B) Scheme of the M protein mutants generated. Numbers below the bars indicate the mutated amino acid (substitution mutants) or flanking amino acids in the deletion mutants. The mutated amino acid or the flanking amino acids of each deletion are indicated above the bars. Substitutions or deletions are indicated by open boxes within bars. Mutant names are indicated in the left column. (C) The mutant genes were expressed in a rabbit reticulocyte lysate in the presence of [35S]methionine/cysteine and were analyzed by SDS-PAGE and autoradiography.